In the framework of the LIDISKI project, a new set of real-time RT-PCR (RT-qPCR) tests to identify virulent and avirulent viruses through pathotype-specific probes has been developed and validated for Newcastle disease (ND).
Test validation was performed according to the World Organisation for Animal Health (WOAH) recommendations and included the testing of a large panel of APMV-1 positive samples of different genotypes originating from Europe, Asia, the Middle East and Africa including those collected under the project from Nigeria.
A new real-time RT-PCR protocol has been published open access in an international journal and is available at the following link. https://doi.org/10.1016/j.jviromet.2023.114813